Identification of Plant Species
Real-time PCR Kits for the Detection of Plant Species
The most comprehensive kit for the screening of plant species is the SpeciesScreen Plant kit, which detects all higher level plant species. One of the possible applications of this kit is the control of extraction efficiency from samples containing plant material.
The identification of plant species is, among other applications, relevant for GMO analysis. A positive result from a GMO screening can occur as a result of a combination of different GMOs in the sample or can be caused by a botanical impurity in the sample. In order to perform cost-efficient and less time consuming follow-up analyses, it is often helpful to narrow down the possibly contained GMOs. This can be done through the exclusion of specific plant species. For the most important plant species, which are currently on the market (such as corn, soy, cotton or rice), Eurofins offers reliable species-specific PCR analyses:
- SpeciesIdent Corn
- SpeciesIdent Soy
- SpeciesIdent Cotton
- SpeciesIdent Rice
Detection of Plant Pathogens
In connection with GMO analyses, the exclusion of natural contamination of the sample with plant pathogens can also be useful. Further information can be found on our site about detection of plant pathogens.
Conventional PCR for the Detection of Plant Species
You will also find kits for conventional PCR in the Eurofins portfolio. Apart from specific corn, soy and cotton analyses, analyses for the plant pathogen cauliflower mosaic virus (CaMV) and Agrobacterium tumefaciens are available.
Are you wondering why we don’t offer multiplex kits for plant species?
There is a good reason for this: Since DNA from plants is very widespread in food and feed samples, the risk of a competition between the PCR systems of a multiplex PCR is rather high. As a consequence, target sequences, which are present at lower concentrations in the sample could be masked by other, higher concentrated ones, resulting in false-negative results.
Therefore, in our opinion, a multiplex PCR is not appropriate for the majority of questions related to the detection of plant DNA in food since the target gene concentration can easily vary by a factor of 104 or more.