Real-time PCR Kits for GMO Screening
Since the first approvals of genetically modified organisms (GMOs) more than 20 years ago, the number of worldwide GMO approvals has increased considerably. Nowadays, food and feed laboratories must take into account over 150 GMOs, in order to guarantee their customers secured analysis results.
Screening methods are essential under these conditions, in order to clarify in an initial analysis step, whether GMO constituents are present in a sample. For some time now, the classic screening tests of the 35S promoter derived from the cauliflower mosaic virus (CaMV 35S promoter) and the nopaline-synthase terminator from Agrobacterium tumefaciens (nos terminator) are no longer sufficient for answering many inquiries.
In order to keep up with the demands of the ever changing approval and cultivation situation, Eurofins constantly develops new screening and multiplex screening methods. These can be combined, depending on the situation – such as country of origin of the raw materials, plant species, sample matrix and, last but not least, your laboratory equipment.
The combination of these three tests is appropriate for many sample matrices. With the application of this classical screening, many GMOs can be either detected or excluded.
Since some GMOs were approved in the last years, which are not detected with this screening method, a combination with other screening methods – especially for soy and rapeseed-containing samples – could be necessary.
- Tests: CaMV 35S promoter, nos terminator, FMV promoter, IPC (internal positive control)
- Validated for: ABI 7500/Fast, LC480, Agilent MX 3000P/3005P, AriaMX and Bio-Rad
Further details can be found in our Productsheet GMOScreen RT 35S/NOS/FMV.
GMOScreen AgroBorder I/II
This screening is a high-performance combination of our new screening methods. It detects multiple GMOs, which are already cultivated, however not detected with the classical 35S/NOS/FMV screening package. In combination with other screening methods, it offers a high coverage for the detection or exclusion of GMOs.
- Tests: AgroBorder I, AgroBorder II, IPC (internal positive control)
- Validated for: ABI GeneAmp® 7500 Fast, Agilent Mx3000P/Mx3005P, AriaMX™, Roche LightCycler® 480 Instrument
Further details can be found in our Productsheet GMOScreen AgroBorder I/II.
GMOScreen RT IPC GM Soy I
This kit was developed especially for the comprehensive detection of genetically modified soy. In addition to the multiplex screenings 35S/NOS/FMV and AgroBorder I/II, it also includes a duplex detection.
- Tests: CaMV 35S promoter, nos terminator, FMV promoter, AgroBorder I, AgroBorder II, CV127, SAMS-als, IPC (internal positive control)
- Validated for: ABI 7500/Fast, Agilent MX3005P/MX3000P and LC48